Monday, June 3 and Tuesday, June 4
This is a two-day wet-lab workshop where participants will dive into purification and analysis of viral vectors and nanoparticles using monolithic columns. The program covers downstream processing procedures including capture and polishing steps, all supported by the PATfix® analytical toolbox for quick and efficient process development. Sartorius BIA Separations experts conduct chromatographic runs using actual samples in a BSL2 laboratory.
What you will learn
- AAV upstream and downstream process optimization with PATfix AAV Switcher monitoring
- Sample preparation and AAV chromatographic purification best practices
- Chromatographic profile differences between monolith ligands (AEX and MM) across AAV serotypes
- Serotype-specific DSP optimization techniques
- AAV subpopulation analysis and quantification using multidetector analytics
- Advanced analysis through ultracentrifuge profiling
- Extracellular vesicle isolation from cell culture with process monitoring
- Adenovirus concentration and purification from host cell impurities
- Endotoxin reduction in bacteriophage purification with analytical tracking